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cell cycle staining kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Structured Review

    Multi Sciences (Lianke) Biotech Co Ltd cell cycle staining kit
    Cell Cycle Staining Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+cycle+staining+kit/Cell+cycle+staining+Kit/custom%40ccs012%4042628379
    Average 98 stars, based on 1479 article reviews
    cell cycle staining kit - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Transfection:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Analysis:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Incubation:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Cell Cycle Assay:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Control:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Oligomer Restriction:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Staining:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    EdU Assay:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    BIA-KA:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Transferring:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Chloramphenicol Acetyltransferase Assay:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Standard Curve:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Lactate Dehydrogenase Assay:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Migration Assay:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a

    Migration:

    Article Title: DDX3X regulates DNA repair pathway choice at double-strand breaks via RNA-DNA hybrid formation.
    Article Snippet: ells per well and transfected with control or DDX3X-targeting siRNAs. At 72 hours posttransfection, cells were harvested and processed for cell cycle analysis using a commercial staining kit (#ccs012, MultiSciences Biotech) according to the manufacturer’s instructions. Briefly, cells were washed with PBS, fixed in icecold ethanol, and stained with DNA staining solution. After incubation for 30min a



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    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
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    Image Search Results


    In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

    Techniques: In Vitro, Fluorescence, Staining